Welcome to Micro-Na, a collaboration between McGill Biodesign and Professor Brambilla’s laboratory at Université de Montréal! Together, we are designing a hydrogel microneedle patch for the non-invasive monitoring of sodium levels.
For our microneedle patch, we selected a commercially available fluorophore called CoroNa Green, which is mostly used in studies of cell behaviour. While there is data available for the ability of CoroNa Green to detect sodium, we wanted to validate it ourselves before moving forward with it. After conducting selectivity and sensitivity tests, the results showed that CoroNa Green is a suitable fluorophore to detect sodium. We could then start on the design experiments!
To fabricate the microneedle patches and combine them with the CoroNa Green fluorophore, we decided to try an existing swelling microneedle composition with potassium or sodium and a composition without. We found that there wasn’t a major difference in how the patch turned out regardless of the composition, so we settled with the formulation that didn’t use sodium or potassium.
We’ve also tested the microneedle patch on pig skin prepared with known parameters to determine the patches’ ability. We noticed that the patches applied to the skin samples with higher levels of sodium had a greater fluorescence intensity. Some future work includes observing the fluorescence for a greater range of sodium levels to establish the microneedles’ accuracy and consistency in detecting sodium levels.
Future of the Project
Lan Anh Hyunh
Lan Anh is currently in U2 Bioengineering and is interested in the fields of biotechnology and machine learning. During her spare time she enjoys playing the violin, archery, and reading.
Zahra is a Team Lead for Micro-Na, where she is involved in the creation of a hydrogel microneedle patch.